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kb extended dna ladder  (New England Biolabs)


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    New England Biolabs kb extended dna ladder
    Kb Extended Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kb+extended+dna+ladder/Quick-Load+1+kb+Extend+DNA+Ladder/pm41385589-544-3-8
    Average 95 stars, based on 93 article reviews
    kb extended dna ladder - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Derivative Assay:

    Article Title: Activation of the ciliary kinase CDKL5 is mediated by the cyclin-dependent kinase CDK20/LF2 to control flagellar length.
    Article Snippet: In each gel, lane 1 is wild-type, lane 2 is lf5-2 and lane 3 is a no DNA added control.In each gel, lane 1 is wild-type, lane 2 is lf5-2 and lane 3 is a no DNA added control.. Ladder is 1 kb extended DNA ladder (N3239, NEB, Ipswich, MA, USA).. In addition to CDKL5, Cre12.g538250, and Cre12.g538200 were also deleted.In addition to CDKL5, Cre12.g538250, and Cre12.g538200 were also deleted.

    Article Title: Methods of producing size-selected nucleic acid libraries and compositions and kits for practicing same
    Article Snippet: 4: Biotin eluate from streptavidin beads after binding with desthiobiotin tagged DNA in lane 3. .. Lane 5: 10 μl of 1 Kb extended DNA ladder, NEB Cat No. N3239S with 0.5 kb, 1 kb, 1.5 kb, 2 kb, 3 kb, 4 kb, 5 kb, 6 kb, 8 kb, 10 kb, 15 kb, 20 kb, 48 kb DNA fragments. .. Lane 6: HindIII digest of 48 kb λ and 48 kb λ post ligation to 5 μl of ds 50mer with desthiobiotin t

    Article Title: Methods of producing size-selected nucleic acid libraries and compositions and kits for practicing same
    Article Snippet: of the lanes on the gel are provided below. .. Lane 1: 10 μl of 1 Kb extended DNA ladder, NEB Cat No. N3239S with 0.5 kb, 1 kb, 1.5 kb, 2 kb, 3 kb, 4 kb, 5 kb, 6 kb, 8 kb, 10 kb, 15 kb, 20 kb, 48 kb DNA fragments. .. Lane 2: ˜2 μg mixture of 1 part 48 kb λ NEB Cat No. N301

    Article Title: Analysis of the transgene insertion pattern in a transgenic mouse strain using long-read sequencing
    Article Snippet: electropherogram was recorded using a laser scanner (FX-Pro, Bio-rad). .. M1: 1 kb extended DNA ladder (NEB), M2: 2-log DNA ladder (NEB). )

    Plasmid Preparation:

    Article Title: Activation of the ciliary kinase CDKL5 is mediated by the cyclin-dependent kinase CDK20/LF2 to control flagellar length.
    Article Snippet: In each gel, lane 1 is wild-type, lane 2 is lf5-2 and lane 3 is a no DNA added control.In each gel, lane 1 is wild-type, lane 2 is lf5-2 and lane 3 is a no DNA added control.. Ladder is 1 kb extended DNA ladder (N3239, NEB, Ipswich, MA, USA).. In addition to CDKL5, Cre12.g538250, and Cre12.g538200 were also deleted.In addition to CDKL5, Cre12.g538250, and Cre12.g538200 were also deleted.

    Article Title: Methods of producing size-selected nucleic acid libraries and compositions and kits for practicing same
    Article Snippet: 4: Biotin eluate from streptavidin beads after binding with desthiobiotin tagged DNA in lane 3. .. Lane 5: 10 μl of 1 Kb extended DNA ladder, NEB Cat No. N3239S with 0.5 kb, 1 kb, 1.5 kb, 2 kb, 3 kb, 4 kb, 5 kb, 6 kb, 8 kb, 10 kb, 15 kb, 20 kb, 48 kb DNA fragments. .. Lane 6: HindIII digest of 48 kb λ and 48 kb λ post ligation to 5 μl of ds 50mer with desthiobiotin t

    Article Title: Methods of producing size-selected nucleic acid libraries and compositions and kits for practicing same
    Article Snippet: of the lanes on the gel are provided below. .. Lane 1: 10 μl of 1 Kb extended DNA ladder, NEB Cat No. N3239S with 0.5 kb, 1 kb, 1.5 kb, 2 kb, 3 kb, 4 kb, 5 kb, 6 kb, 8 kb, 10 kb, 15 kb, 20 kb, 48 kb DNA fragments. .. Lane 2: ˜2 μg mixture of 1 part 48 kb λ NEB Cat No. N301

    Article Title: Analysis of the transgene insertion pattern in a transgenic mouse strain using long-read sequencing
    Article Snippet: electropherogram was recorded using a laser scanner (FX-Pro, Bio-rad). .. M1: 1 kb extended DNA ladder (NEB), M2: 2-log DNA ladder (NEB). )

    Sequencing:

    Article Title: Activation of the ciliary kinase CDKL5 is mediated by the cyclin-dependent kinase CDK20/LF2 to control flagellar length.
    Article Snippet: In each gel, lane 1 is wild-type, lane 2 is lf5-2 and lane 3 is a no DNA added control.In each gel, lane 1 is wild-type, lane 2 is lf5-2 and lane 3 is a no DNA added control.. Ladder is 1 kb extended DNA ladder (N3239, NEB, Ipswich, MA, USA).. In addition to CDKL5, Cre12.g538250, and Cre12.g538200 were also deleted.In addition to CDKL5, Cre12.g538250, and Cre12.g538200 were also deleted.

    Article Title: Methods of producing size-selected nucleic acid libraries and compositions and kits for practicing same
    Article Snippet: 4: Biotin eluate from streptavidin beads after binding with desthiobiotin tagged DNA in lane 3. .. Lane 5: 10 μl of 1 Kb extended DNA ladder, NEB Cat No. N3239S with 0.5 kb, 1 kb, 1.5 kb, 2 kb, 3 kb, 4 kb, 5 kb, 6 kb, 8 kb, 10 kb, 15 kb, 20 kb, 48 kb DNA fragments. .. Lane 6: HindIII digest of 48 kb λ and 48 kb λ post ligation to 5 μl of ds 50mer with desthiobiotin t

    Article Title: Methods of producing size-selected nucleic acid libraries and compositions and kits for practicing same
    Article Snippet: of the lanes on the gel are provided below. .. Lane 1: 10 μl of 1 Kb extended DNA ladder, NEB Cat No. N3239S with 0.5 kb, 1 kb, 1.5 kb, 2 kb, 3 kb, 4 kb, 5 kb, 6 kb, 8 kb, 10 kb, 15 kb, 20 kb, 48 kb DNA fragments. .. Lane 2: ˜2 μg mixture of 1 part 48 kb λ NEB Cat No. N301

    Article Title: Analysis of the transgene insertion pattern in a transgenic mouse strain using long-read sequencing
    Article Snippet: electropherogram was recorded using a laser scanner (FX-Pro, Bio-rad). .. M1: 1 kb extended DNA ladder (NEB), M2: 2-log DNA ladder (NEB). )

    Agarose Gel Electrophoresis:

    Article Title: Activation of the ciliary kinase CDKL5 is mediated by the cyclin-dependent kinase CDK20/LF2 to control flagellar length.
    Article Snippet: In each gel, lane 1 is wild-type, lane 2 is lf5-2 and lane 3 is a no DNA added control.In each gel, lane 1 is wild-type, lane 2 is lf5-2 and lane 3 is a no DNA added control.. Ladder is 1 kb extended DNA ladder (N3239, NEB, Ipswich, MA, USA).. In addition to CDKL5, Cre12.g538250, and Cre12.g538200 were also deleted.In addition to CDKL5, Cre12.g538250, and Cre12.g538200 were also deleted.

    Article Title: Methods of producing size-selected nucleic acid libraries and compositions and kits for practicing same
    Article Snippet: 4: Biotin eluate from streptavidin beads after binding with desthiobiotin tagged DNA in lane 3. .. Lane 5: 10 μl of 1 Kb extended DNA ladder, NEB Cat No. N3239S with 0.5 kb, 1 kb, 1.5 kb, 2 kb, 3 kb, 4 kb, 5 kb, 6 kb, 8 kb, 10 kb, 15 kb, 20 kb, 48 kb DNA fragments. .. Lane 6: HindIII digest of 48 kb λ and 48 kb λ post ligation to 5 μl of ds 50mer with desthiobiotin t

    Article Title: Methods of producing size-selected nucleic acid libraries and compositions and kits for practicing same
    Article Snippet: of the lanes on the gel are provided below. .. Lane 1: 10 μl of 1 Kb extended DNA ladder, NEB Cat No. N3239S with 0.5 kb, 1 kb, 1.5 kb, 2 kb, 3 kb, 4 kb, 5 kb, 6 kb, 8 kb, 10 kb, 15 kb, 20 kb, 48 kb DNA fragments. .. Lane 2: ˜2 μg mixture of 1 part 48 kb λ NEB Cat No. N301

    Article Title: Analysis of the transgene insertion pattern in a transgenic mouse strain using long-read sequencing
    Article Snippet: electropherogram was recorded using a laser scanner (FX-Pro, Bio-rad). .. M1: 1 kb extended DNA ladder (NEB), M2: 2-log DNA ladder (NEB). )



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    Schematic of MASTR-seq procedure Day 1: (Step 1) Samples are dissociated to single-cell suspension, and cells are lysed. RNA and protein are digested by RNase A and proteinase K, respectively. DNA is subsequently purified by commercial HMW gDNA extraction kit or phenol:chloroform:isoamyl alcohol (PCIA, 25:24:1)-based method. (Step 2) HMW genomic DNA quality control assays are performed using Nanodrop for DNA quality, Qubit for quantifying DNA concentration, and gel electrophoresis for DNA integrity. Day 2: crRNA/tracrRNA annealing and Cas9 RNP assembling are completed (not shown in this schematic) before (step 3) HMW DNA dephosphorylation, (step 4) Cas9-mediated RNP cleavage, and (step 5) dA-tailing. (Step 6) The quality of Cas9-mediated RNP cleavage efficiency is assessed by PCR amplification of the target region using flanking primers. (Step 7) Cas9-RNP-cleaved products are purified from salts using sodium acetate-ethanol precipitation. Days 2–3: (step 8) target DNA fragments are precisely size-selected using BluePippin. Day 4: (step 9) samples are barcoded in a PCR-free manner and pooled for multiplexing using nanopore native barcodes. (Step 10) Adapters are ligated onto the multiplexed DNA. Days 4–6: (step 11) DNA is loaded onto the Nanopore flow cell and sequenced for 48 h (Step 12) Raw data are processed by basecalling, aligning and demultiplexing sequentially using Dorado. Custom computational tools are employed for the analyses of single-allele DNA methylation and STR tract length. See also .

    Journal: Cell Reports Methods

    Article Title: MASTR-seq enables multiplexed analysis of short tandem repeats with sequencing

    doi: 10.1016/j.crmeth.2026.101341

    Figure Lengend Snippet: Schematic of MASTR-seq procedure Day 1: (Step 1) Samples are dissociated to single-cell suspension, and cells are lysed. RNA and protein are digested by RNase A and proteinase K, respectively. DNA is subsequently purified by commercial HMW gDNA extraction kit or phenol:chloroform:isoamyl alcohol (PCIA, 25:24:1)-based method. (Step 2) HMW genomic DNA quality control assays are performed using Nanodrop for DNA quality, Qubit for quantifying DNA concentration, and gel electrophoresis for DNA integrity. Day 2: crRNA/tracrRNA annealing and Cas9 RNP assembling are completed (not shown in this schematic) before (step 3) HMW DNA dephosphorylation, (step 4) Cas9-mediated RNP cleavage, and (step 5) dA-tailing. (Step 6) The quality of Cas9-mediated RNP cleavage efficiency is assessed by PCR amplification of the target region using flanking primers. (Step 7) Cas9-RNP-cleaved products are purified from salts using sodium acetate-ethanol precipitation. Days 2–3: (step 8) target DNA fragments are precisely size-selected using BluePippin. Day 4: (step 9) samples are barcoded in a PCR-free manner and pooled for multiplexing using nanopore native barcodes. (Step 10) Adapters are ligated onto the multiplexed DNA. Days 4–6: (step 11) DNA is loaded onto the Nanopore flow cell and sequenced for 48 h (Step 12) Raw data are processed by basecalling, aligning and demultiplexing sequentially using Dorado. Custom computational tools are employed for the analyses of single-allele DNA methylation and STR tract length. See also .

    Article Snippet: Check integrity of HMW DNA on a 0.6% 1X TAE agarose gel with 1 kb Extend DNA ladder (NEB#N3239S).

    Techniques: Single Cell, Suspension, Purification, Extraction, Control, Concentration Assay, Nucleic Acid Electrophoresis, De-Phosphorylation Assay, Amplification, Ethanol Precipitation, Multiplexing, DNA Methylation Assay